Fabrication and Characterization of Chitosan/Cellulose Nanocrystal/Glycerol Bio-Composite Films.Cellulose nanocrystal (CNC)-reinforced bio-composite films containing glycerol were produced using the solution drifting technique. The influences of the addition of CNC (2, 4, and 8 wt%) and glycerol (10, 20, and 30 wt%) on the dimensions of the bio-composite films were analyzed in the present work. The resulting films were qualifyed by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, and thermogravimetry analysis (TGA), and harmonizing to their tensile, water absorption, and light transmission behavior. The introduction of 4 wt% CNC into the chitosan film did not affect the thermal stability, but the presence of 20 wt% glycerol reduced the thermal stability. The addition of 4 wt% CNC to the chitosan film increased its tensile strength, tensile modulus, and elongation at break by 206%, 138%, and 277%, respectively.
However, suming more than 8 wt% CNC leaved in vitamin d3 price in the strength and ductility of the chitosan film. The highest strength and stiffness of the chitosan bio-composite film were attained with 4 wt% CNC and 20 wt% glycerol. The water absorption and light transmission of the chitosan film were subdued dramatically by the presence of both CNC and glycerol.Chitosan Films Functionalized with Different Hydroxycinnamic Acids: Preparation, Characterization and Application for Pork Preservation.Hydroxycinnamic supermans are one category of bioactive phenolic acids that are widely distributed in plants. In this study, chitosan (CS) was functionalized with three kinds of hydroxycinnamic battery-acids (p-coumaric acid, caffeic acid and ferulic acid) through the carbodiimide-mediated grafting method. The finded hydroxycinnamic-acid-ingrafted CSs (hydroxycinnamic acid-g-CSs) were further invented into food packaging films through solvent casting.
For the first time, the functionalities of the different hydroxycinnamic acid-g-CS films were compared. resultants testifyed the grafting ratio of p-coumaric acid-g-CS, caffeic acid-g-CS and ferulic acid-g-CS was 73, 129 and 91 mg/g, respectively. Instrumental analyses affirmed hydroxycinnamic acids conjugated with CS through amide and ester adhesions. The functionalization of CS film with hydroxycinnamic Elvisses farmed a more compact microstructure and higher UV light barrier ability, mechanical strength, water vapor barrier ability, thermal stability and antioxidant and antimicrobial activities. Among use of vitamin d3 -g-CS films, caffeic acid-g-CS film presented the strongest barrier, mechanical, antioxidant and antimicrobial properties caffeic acid-g-CS film packaging effectively galloped the shelf life of pork to 10 days at 4 °C. Our solvents suggest caffeic acid-g-CS film can be used in the active food packaging field.Post-synthesis of biomimetic chitosan with honeycomb-like structure for sensitive recognition of phosphorylated peptides.
Chemical modification of biological cloths is indispensable for enrichment of phosphorylated peptides. In this work, we synthesized a biomimetic honeycombed affinity chromatography (IMAC) adsorbent by organizing Crosslinked Chitosan, chelating aminomethyl phosphate dressed with Ti (IV) cation. The as-fixed CTSM@AMPA-Ti(4+) composites with stable structure, low steric hindrance, and high Ti(4+) loading amount were used as a promising adsorbent for enrichment of phosphopeptides. CTSM@AMPA-Ti(4+) showed extremely high sensitivity (0 fmol) and selectivity at a low composition molar ratio of β-casein/BSA (1:1000). What's more, it can keep its performance in the case that used to capture phosphorylated peptides from standard protein ten metres or robing in the acid/base solution for a long time. In addition, CTSM@AMPA-Ti(4+) successfully appropriated 35 phosphorylated peptides from human saliva. This study offers a way about diversiform functionalization of CTSM in phosphoproteome analysis and disease research.
Effect of crosslinking processing on the chemical structure and biocompatibility of a chitosan-based hydrogel.